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Accueil > Animation Scientifique > Année 2013

Séminaire Nathalie Reuter

Le vendredi 15 novembre à 11h en Salle Jean Barriol,
Faculté des Sciences et Technologies, Entrée 2A, Niveau 7,
le Pr. Nathalie Reuter, Université de Bergen (Norvège)
Computational Biology Unit and Department of Molecular Biology
- contact : G.Monard : Gerald.Monard@univ-lorraine.fr -
 
donnera une conférence intitulée :
 
"How do water-soluble proteins stick to membranes ? Some answers from molecular simulations of amphitropic proteins
 

Abstract

Unlike integral membrane proteins, such as channels or pumps, amphitropic proteins do not span the lipid bilayer but bind at the surface of cell or organelle membranes to perform their function. Their binding is reversible and often specific towards certain types of lipids.
I will start by giving an introduction on amphitropic proteins as well as the structural and energetic aspects of their binding to membranes. Next I will present the work performed in our group on a phosphatidylinositol-specific phospholipase C (PI-PLC) to illustrate how molecular dynamics simulations at different levels of resolution can be used to get a more detailed picture of the interactions between amphitropic proteins and membrane lipids. The in silico results will be discussed in light of the experimental dissociation constants (Kd) provided by our collaborators, who measured Kds of wild type PI-PLC and mutants on small unilamellar vesicles using fluorescence correlation spectroscopy. Using both approaches, we could show the importance of ’pi-cation’ interactions between tyrosines and choline from the lipid headgroups.
 

Agenda

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